Extended Data Fig. 4: The C-terminal motif of DltX, and particularly its invariant tyrosine residue, is required for S. aureus growth on tunicamycin.
From: Mechanism of d-alanine transfer to teichoic acids shows how bacteria acylate cell envelope polymers
a, Full set of controls for the spot titer assay shown in Fig. 3b. S. aureus strains were grown on TSA plates containing the indicated compounds: DMSO at 1.25 µL per 1.00 mL of TSA, IPTG at 1.00 mM, anhydrotetracycline (aTc) at 0.4 µM, and tunicamycin at 1.0 µg/mL. Tunic. inhibits wall teichoic acid biosynthesis, IPTG was used to induce expression of the indicated plasmid-borne cassette (here, null refers to a strain with an empty IPTG-inducible cassette in the plasmid), aTc was used to induce expression of the indicated chromosomally integrated cassette, and DMSO was used as a vehicle control for the tunic. The image of the plate with tunic., IPTG, and aTc has been reproduced here for clarity. flag-dltX∆motif encodes for an N-terminally FLAG-tagged DltX variant lacking the last six amino acids. b, Full set of controls for the spot titer assay shown in Fig. 3c. Compounds were used at the same concentrations as above, for the same reasons as above. Here, the bracketed sequences in the superscripts of the aTc-inducible cassettes represent the C-terminal motif sequence of the given DltX variant. Red letters denote changes from the wild-type sequence.
