Fig. 3: A model of the Sin Nombre glycoprotein lattice in complex with Fab SNV-42.

a, An EM-derived model of a Sin Nombre virion decorated in Fab fragments of mAb SNV-42. The virion model is derived from previously reported cryoET data of Tula virus⁵. The Gc is coloured blue and the Gn coloured pink or purple for the head or stalk regions, respectively. The light or heavy chains of the Fab are coloured light or dark grey, respectively. The zoomed inset displays nine individual glycoprotein spikes with the central spike surface rendered at higher resolution. The Fab fragments bound to the central spike are displayed as a backbone trace. b, Top view (left) and side view (right) of the Sin Nombre glycoprotein spike bound to Fab fragments of SNV-42. This assembly model is based on the previously reported ANDV glycoprotein spike tetramer (PDB: 6ZJM). The location of two SNV-42 escape mutants (T312K and K357Q) are displayed in orange and the equivalent locations of other previously reported antibody escape mutants are displayed in red. The complete list of antibody escape mutants and the species they apply to are detailed in Supplementary Table 2. To enable visualization of all epitopes, two loops that are not resolved in this SNV Gn structure (residues 86–99 and 221–229) were replaced by their equivalents from a previously reported ANDV Gn structure (PDB: 6ZJM). c, The equivalent view of a hantavirus glycoprotein spike bound to Fab HTN-Gn1, a previously reported neutralizing antibody that binds to HNTV¹⁴.