Extended Data Fig. 5: DENV infection in splenic macrophage populations.
From: Human FcγRIIIa activation on splenic macrophages drives dengue pathogenesis in mice
(a) DENV infection (DENV+) of macrophage populations in the spleen of Ifnar1−/−/FcγR humanized mice that were pre-treated with the Fc-variants (GRLR, WT or ALIE) of the anti-DENV 2D22 was determined 4 days post infection. Representative flow cytometry histograms and quantification of each population are presented. Marginal zone (MZ) macrophages were defined as CD11b+/F4/80+/Tim4high. MZ metallophilic macrophages were defined as CD11b+/F4/80+/Tim4low. Red pulp macrophages were defined as CD11blow/F4/80+. Groups were compared by one-way ANOVA (Bonferroni post hoc analysis adjusted for multiple comparisons). n = 4 mice per group. In box plots, the center line shows the median, boxes represent the middle quartiles and whiskers show the range of values (minimum to maximum).
