Extended Data Table 1 Metabolic concentrations (μM) detected using NMR

From: Drought re-routes soil microbial carbon metabolism towards emission of volatile metabolites in an artificial tropical rainforest

Compound

Pre-drought

Drought

Name

Formula

P15-0hr

P24-0hr

P26-0hr

P34-0hr

P35-0hr

P12-Ctrl

P15-0hr

P24-6hr

P26-0hr

P34-0hr

P34-0hr

2-Oxoisocaproate**P

C6H10O3

6

5

D

5

D

ND

ND

ND

ND

ND

D

3-Hydroxybutyrate

C4H8O3

ND

ND

ND

ND

D

ND

ND

ND

ND

ND

ND

Acetate

C2H4O2

22

38

30

55

26

19

35

31

31

24

11

Acetone

C3H6O

ND

D

D

ND

ND

D

ND

ND

ND

ND

D

Alanine**P

C3H7NO2

24

21

15

20

17

18

9

13

9

7

9

Benzoate

C7H6O2

ND

ND

ND

D

ND

ND

ND

ND

ND

ND

ND

Betaine

C5H11NO2

ND

ND

ND

ND

ND

D

D

ND

ND

ND

ND

Ethanol

C2H6O

12

17

21

19

18

28

24

12

22

22

12

Formate***P

CH2O2

17

38

40

23

31

ND

D

6

16

D

11

Glutamate

C5H9NO4

12

12

8

6

10

11

7

10

10

10

6

Glycerol

C3H8O3

6

8

8

6

7

5

6

7

5

8

6

Glycine*P

C2H5NO2

12

9

6

8

7

5

D

7

6

D

D

Isoleucine

C6H13NO2

7

7

D

D

D

D

D

D

D

D

D

Lactate

C3H6O3

6

7

D

12

7

D

D

11

D

D

7

Leucine*P

C6H13NO2

12

11

7

7

6

7

6

6

6

D

D

Methanol

CH4O

150

375

367

222

326

508

329

203

370

372

322

Phenylalanine*P

C9H11NO2

D

D

ND

D

ND

ND

ND

ND

ND

ND

ND

Pyroglutamate*P

C5H7NO3

22

19

10

16

9

8

5

12

8

D

D

Pyruvate**P

C3H4O3

11

13

10

11

10

D

5

9

5

D

7

Succinate

C4H6O4

ND

ND

ND

ND

ND

ND

D

D

ND

ND

ND

Threonine

C4H9NO3

6

7

D

D

D

D

D

D

D

D

D

Trehalose*D

C12H22O11

ND

ND

ND

ND

ND

18

D

7

6

13

D

Uracil*P

C4H4N2O2

D

5

D

D

D

ND

ND

D

ND

ND

D

Valine*P

C5H11NO2

15

13

8

9

8

8

6

8

6

5

5

  1. Samples displayed are a subset of total samples based on the quality of NMR data. Most samples are from 0 h, except for P24 during drought which is from 6 h post pyruvate injection.
  2. ND, not detected (below the level of detection where concentration < 1.9 μM); D, detected (below level of quantification, where concentration 2 - 5 μM)
  3. P, Compound higher during pre-drought (Linear Fixed Effect Model (d.f. = 7), where ND and D were given values of 1 and 4 μM respectively, Oxoisocaproate P=0.0014, Alanine P=0.0074, Formate P=4.2E-4, Glycine P=0.017, Leucine P=0.016, Phenylalanine P=0.030, Pyroglutamate P=0.018, Pyruvate P=0.0011, Uracil P=0.022, Valine P=0.012)
  4. D, Compound higher during drought (Trehalose P=0.020)
  5. *, P<0.05; **, P<0.01; ***, P<0.001
Back to article page