Extended Data Fig. 7: In vitro evaluation of spacer integration into four CRISPR arrays in the presence or absence of P. furiosus histones.
From: Histones direct site-specific CRISPR spacer acquisition in model archaeon
(a) Gel images show representative results from PCR (carried out as in Fig. 5d) with primers targeting four CRISPR arrays: CRISPR5, CRISPR6, CRISPR7, and CRISPR8. The expected sizes for PCR products resulting from integrations at repeats 1 - 5 are marked with R1 – R5, respectively. Red asterisks identify the band corresponding to integrations at repeat 1, which is the natural, preferred point of integration in vivo. (b) Intensity of all PCR bands was quantified using ImageJ and the proportion of integration events at the five repeats was determined. Single biological replicates were evaluated with two different primer pairs producing with similar outcomes; one PCR reaction is shown and quantitated here.
