Extended Data Fig. 2: Distributions of aligned DNA sequences from MNase protection assay.
From: Histones direct site-specific CRISPR spacer acquisition in model archaeon
MNase protected DNA (Fig. 1a) was sequenced by HTS, aligned, and genome browser tracks were generated to examine the genome-wide distribution of aligned DNA fragments. Overlapping subtracks are color-coded according to the size of the DNA fragment (red: 30 bp +/- 5; golden yellow: 60 bp +/- 5; green: 90 bp +/- 5; blue 120 bp +/- 5). Positions of CRISPR loci are indicated (leader in pale yellow, repeat array in black). Approximately 10 kb of genome space upstream and downstream from each CRISPR locus is shown. Six replicates (three stationary growth, three exponential) were sequenced and yielded similar patterns; a representative replicate is shown here.
