Fig. 2: Mutants of BPs∆33HTH_HRM10 and Muddy overcome TPP loss.
From: Therapeutically useful mycobacteriophages BPs and Muddy require trehalose polyphleates
a, Tenfold serial dilutions of BPs∆33HTH_HRM10 and gp22 mutants (as indicated on the left; see Extended Data Table 2) were spotted onto solid media with M. smegmatis mc2155, M. abscessus GD01 or M. abscessus GD01 transposon insertion mutant strains. Plaque assays were performed at least twice with similar results. The locations of amino acid substitutions in BPs∆33HTH_HRM10 gp22 conferring the ability to infect TPP-deficient strains (red) or previously found to broaden host range to include M. tuberculosis (purple) (bottom panel) are indicated. The A306V and A604E substitutions were identified with both assays. b, Adsorption of phages BPs∆33HTH_HRM10, phKSW1 and ZoeJ∆43–45 to M. abscessus strains GD01 and GD01 fadD23::Tn (GD01Tn_BPs_HRM10_RM1) as indicated by the percentage of unadsorbed phages remaining in infection supernatants at different times after infection. Assays were performed in duplicate twice and data presented are mean ± s.d. c, Tenfold serial dilutions of Muddy and Muddy gp24 mutants (as indicated on the left) were spotted onto solid media with M. smegmatis mc2155, M. abscessus GD01 or M. abscessus GD01 transposon insertion mutant strains. Plaque assays were performed at least twice with similar results. The locations of amino acid substitutions in Muddy gp24 that confer the ability to infect TPP-deficient strains (bottom panel) are indicated.
