Fig. 5: TPPs are also required for infection of M. smegmatis by phages BPs and Muddy.

a, Tenfold serial dilutions of phages (as indicated on the left) were spotted onto solid media with M. smegmatis mc²155, ∆pks, ∆papA3 or ∆fadD23 as indicated. Plaque assays were performed at least twice with similar results. b, TLC analysis of total lipids extracted from wild-type M. smegmatis, three TPP-deficient mutants and the corresponding complemented strains. Eluent: CHCl₃/CH₃OH (90:10 v/v). TLC was revealed by spraying anthrone on the plate, followed by charring. c, Adsorption of phages BPs∆33HTH_HRM10, phKSW1 and ZoeJ∆43–45 to M. smegmatis strains mc²155 and ∆pks as indicated by the percentage of unadsorbed phages remaining in the supernatant at different times after infection. Assays were performed in triplicate at least twice with similar results and a representative experiment is shown. Data are represented as mean ± s.d. Other replicates are shown in Extended Data Fig. 5. d, A panel of phages from various genetic clusters were tenfold serially diluted and spotted onto M. smegmatis mc²155 and ∆pks. Phage names are shown with their cluster/subcluster designation in parentheses. Plaque assays were performed twice with similar results.