Extended Data Fig. 3: Characterization of TPP-defective mutants.
From: Therapeutically useful mycobacteriophages BPs and Muddy require trehalose polyphleates
A. Ziehl-Neelsen staining of M. abscessus TPP mutants. Cultures of GD22, GD22_RM, GD22_RM::C, GD180, GD180_RM and GD180_RM::C were fixed on glass slides and acid-fast staining was performed using the BD Carbolfuchsin kit, and observed microscopically at 60x magnification. This assay was performed three times. Scale bar: 30 µm. B. MIC (in µg/mL) values of antibiotics determined in Cation-adjusted Mueller-Hinton Broth (CaMHB) at 30 °C against M. abscessus clinical isolates (GD22 and GD180), resistant mutants (GD22_RM4 and GD180_RM2) and complemented strains (GD22_RM4::C and GD180_RM2::C) with CIP104536 (ATCC19977) as a control. Results from three independent experiments are shown.
