Extended Data Fig. 6: Absence of hibernation factors in the S. lophii single particle map.
From: CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state
The single particle map of the S. lophii ribosome shows no densities for hibernation factors found in the microsporidian species V. necatrix and P. locustae. a, b Atomic model of the V. necatrix ribosome (multicolour) fitted into the density map of S. lophii (transparent grey; L1 stalk density shown as green surface and tRNA density shown as beige surface). The hibernation factor MDF1 blocking the mRNA and tRNA binding sites (green ribbon) is shown in a and the hibernation factor MDF2 occupying the polypeptide exit tunnel (PET) is shown with the same colour in b. c, d Close-up views of hibernation factors MDF1 and MDF2 from the V. necatrix structure. e, f Atomic model of the P. locustae ribosome (multicolour) fitted into the density map of S. lophii (transparent grey; L1 stalk density shown as green surface and tRNA density shown as beige surface). The hibernation factor Lso2 is shown as a green ribbon, occupying the tRNA P- site in e. g, h Close-up views of hibernation factor Lso2 from the P. locustae structure.
