Fig. 5: Phylogenetic analyses of Glp in Actinobacteria.

a, Maximum-likelihood phylogeny of MoeA homologues in Actinobacteria. The clade with a blue background corresponds to Glp, clades in pink correspond to other MoeA homologues. Monophyletic classes were collapsed into a single branch for clarity. Dots indicate ultrafast bootstrap (UFB) > 0.85. Scale bar, average number of substitutions per site. For the detailed tree, see Supporting Data. The genomic context of glp in Cglu and M. tuberculosis is indicated on the right of the tree. The locus tags are indicated for genes glp and glpr present in Cglu (Cgl locus tag) and M. tuberculosis (Rv locus tag) genomes. b, Partial alignment of three selected regions from MoeA paralogues in Corynebacteriales. Sequences of Glp and MoeA are shown for the same species, representative of all Corynebacteriales families. The FtsZ-binding loop is delimited by the key residues methionine (M361) and tyrosine (Y369) indicated according to their position on the Cglu sequence. The Pro-rich hinge regions 1 and 2 are indicated by a red rectangle and the first residue inside the box is numbered and highlighted above. c, Phyletic pattern for the presence of MoeA, Glp and GlpR in Actinobacteria. Full circles indicate presence of the gene in >50% of the analysed genomes of the phylum. Column MoeA indicates the presence of one (light pink) or more (dark pink) paralogues, except for Glp that is indicated in a separate column. The presence of GlpR is indicated by yellow dots. The phyletic pattern is represented on a reference Actinobacteria tree. Actinobacteria classes were collapsed into a single branch for clarity. Actinomycetes class is indicated by a dashed line. Dots indicate UFB > 0.85. Scale bar, average number of substitutions per site. For the detailed tree, see Extended Data Fig. 7, and for the detailed analysis, see Supplementary Table 3.