Extended Data Fig. 9: Evaluation of cell death in Mtb-infected and bystander BMDM at an MOI of 10.
From: BACH1 promotes tissue necrosis and Mycobacterium tuberculosis susceptibility
Bach1-/- and WT BMDMs were infected with RFP-labeled H37Rv (H37Rv-RFP) Mtb at an MOI of 10 and cell death, lipid peroxidation and Gpx4 expression assessed at day 1 p.i. (A) Frequencies of Mtb-infected (Mtbp°s) and bystander (Mtbneg) BMDMs. (B) Frequency of Live/Deadhigh cells within total CD11b+ cells. (C) Lipid peroxidation (evaluated by LAA staining) and (D) Gpx4 protein expression analyzed by flow cytometry. The data represent means ± SEM of triplicate samples and are representative of two independent experiments. Statistical significance was assessed by one-way ANOVA for the indicated experimental conditions. (ns, not significant).
