Fig. 6: B6.Sst1^SBach1^−/− mice exhibit enhanced host resistance to Mtb infection.

a–e, B6.Sst1^S and B6.Sst1^SBach1^−/− mice were infected at low dose with Mtb. a, Survival curves of Mtb-infected B6.Sst1^S (n = 44) and B6.Sst1^SBach1^−/− (n = 33) mice. Data pooled from 4 independent experiments (P < 0.0001, Mantel–Cox test). b, C.f.u.s determined at 35 dpi by plating lung and spleen homogenates onto 7H11 agar plates. Results are pooled from 3 separate experiments (WT n = 15 vs Bach1^−/− n = 18 for lungs and WT n = 10 vs Bach1^−/− n = 10 for spleens; two-tailed Mann–Whitney U-test). c, Representative H&E (purple) and ZN (blue) images of lungs from B6.Sst1^S (top) and B6.Sst1^SBach1^−/− (bottom) mice. Each image is a composite of sections from 4–6 individual mice per group per experiment (n = 13–16 total, 3 experiments performed; two-tailed Mann–Whitney U-test). As indicated, B6.Sst1^S mice exhibited massive necrotic tissue damage (asterisk) with intrabronchial and intra-alveolar (arrow) accumulation of necrotic cellular material. B6.Sst1^SBach1^−/− mice also showed markedly reduced tissue necrosis (arrow). d,e, Parenchymal enlargement (d) and TB lesion areas (e) as measured in the lung sections. Data pooled from 3 independent experiments (B6.Sst1^S n = 16 vs B6.Sst1^SBach1^−/− n = 13; two-tailed Mann–Whitney U-test). Data shown in a, b, d and e represent mean ±s.e.m.

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