Extended Data Fig. 7: Digital PCR analysis of the oriC copy number relative to terC in E. coli recipient strain during a conjugation mimicking assay.
From: Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites
DNA extraction and PCR were performed at 0, 30, 60, 180 minutes (min) and at an overnight (O/N) time of incubation after the beginning of the conjugation. Bar charts show the mean of three independent experiments (n = 3, individual plots) and error bars show the standard deviation. Grey asterisk (*) indicates the recombination frequency was below detection level.
