Extended Data Fig. 9: Determination of the attG recombination nature.
From: Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites
a, Single or double cleavage of attG sites?. Double cleavage: we expect heterogeneity of products due to the repair process. Single cleavage: we expect homogeneity of products due to the replication process. b, Bottom or top strand cleavage of attG sites?. Bottom strand cleavage: we expect an orientation + (light brown arrow) and a functional attC site. Top strand cleavage: we expect an orientation - (dark brown arrow) and a non-functional attC site. attG containing replicons are represented by dark lines and attC cassettes by grey ones. The precise cleavage point is indicated by a red arrow. ss and ds: single and double-stranded; bs: bottom strand.
