Extended Data Fig. 6: The mechanism by which Mig1 affects glucose-induced AmB tolerance revealed by time-series transcriptome analyses.
From: Brain glucose induces tolerance of Cryptococcus neoformans to amphotericin B during meningitis
a, Percent survival of C. neoformans wildtype and mig1Δ cells incubated with AmB at 10 × MIC in YP broth with 2% glucose (Glu) or 2% galactose (Gal). Wildtype and mig1Δ cells with or without AmB treatment for 10, 20, or 30 minutes were collected for further transcriptome analyses. Data represent the mean ± SD (n = 3). b, Principal component analysis (PCA) of dynamic transcriptome data of C. neoformans wildtype and mig1Δ cells treated with AmB (10 × MIC). Each plot represents the mean from three biological repeats. c, Plotting the 7 dimensions determined to be statistically significant by permutation analysis. d, Pearson correlations of metabolite alteration, Mig1 function and AmB treatment to dimensions 1–7. e, Biological functional enrichment analysis of genes specifically regulated by Mig1 in response to glucose. f, Fold change of genes related to ergosterol synthesis under different conditions. Data shown in the figure are from the AmB-untreated samples. Two-sided Wald test in DESeq2 in (f).
