Extended Data Fig. 4: The effects of C. minuta on DIO mice.
From: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids
a, The ileal leptin; b, Plasma low density lipoprotein cholesterol (LDL); c, Liver’s index; d, Plasma aspartate amino transferase (AST); e, Plasma alanine aminotransferase (ALT); f, Hepatic aspartate amino transferase (AST); g, Hepatic alanine aminotransferase (ALT); h, The Plasma lipopolysaccharide (LPS); i, Ileum glucagon-like peptide 1 (GLP-1); j, Colonic GLP-1; k, The quantification of bile acids in ileal contents; l, The transcription level of bile acid receptor genes in ileum; m, The transcription level of bile acid receptor genes in colon; n, The transcription level of genes involved in hepatic circadian clock; o, The transcription level of bile acid related genes in liver. For panel k - o, the group names were marked with different color bars in the panel. DIO_CK group: DIO mice were fed with HFD and daily administrated with PBS buffer, n = 5 biologically independent samples; DIO_ND group: DIO mice (purchased from vendor) were fed with low fat normal control diet and daily administrated with PBS buffer for 8 weeks, n = 5 biologically independent samples; C57BL/6J group: normal C57BL/6J mice were fed with normal chow diet and daily administrated with PBS buffer, n = 5 biologically independent samples; DIO_CM group: DIO mice were fed with HFD and daily administrated with C. minuta cells (5*108 CFU), n = 10 biologically independent samples. For panel a, c, d, e, h, i and j, one-Way ANOVA test followed by Dunnett’s multiple comparisons test was performed for statistical analysis of difference between DIO_CK (the control group) and the other groups; For panel b, f, g, l, m, n and o, Kruskal-Wallis test followed by Dunn’s multiple comparisons test was performed for statistical analysis of difference between DIO_CK (the control group) and the other groups. Symbols: **** indicates P value < 0.0001, *** indicates P value < 0.001, ** indicates P value < 0.01, * indicates P value < 0.05. Data were shown as the mean ± SEM unless otherwise indicated.
