Extended Data Fig. 5: The effects of C. minuta on host metabolism and metabolically-important hormones in DIO mice.
From: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids
a, The averaged food consumption of each mouse per day; b, The changes of body weight during 7-week treatment; c, Free-diet blood glucose; d, Fasted-diet blood glucose; e and f, Blood glucose profile(e) and the AOC (f) calculated during the oral glucose tolerance test (OGTT); g and h, Blood glucose profile (g) and the aera of the curve (AOC) (h) calculated during the insulin tolerance test (ITT); i, Plasma triglycerides (TG) j, Plasma total cholesterol (TCHO); k, Active GLP-1 stimulus-secretion test; l, Serum insulin secretion test; m, Plasma glucagon; n, Glucose dependent insulin stimulating peptide (GIP); o, Plasma leptin; p, Plasma ghrelin. The physiological and biochemical indexes in panel c-l were measured using venous blood collected before mouse execution while the others were measured after the end-point execution and sampling of mice after 7-week treatments. DIO_CKrepeat group: DIO mice fed with HFD and daily administrated with PBS buffer for 7 weeks, n = 6 biologically independent samples; DIO_CMrepeat group: DIO mice fed with HFD and daily administrated with C. minuta cells (2*109 CFU) for 7 weeks, n = 6 biologically independent samples. C57BL/6Jrepeat group: normal C57BL/6J mice fed with normal control diet and daily administrated with PBS buffer for 7 weeks, n = 5 biologically independent samples; For panels a, Welch’s ANOVA test followed by two-sided Dunnett’s T3 multiple comparisons test was performed for statistical analysis of difference between DIO_CKrepeat (the control group) and the other groups; For panel b, d, f, g, h, i, j, l, and m, one-Way ANOVA test followed by Dunnett’s multiple comparisons test was performed for statistical analysis of difference between DIO_CKrepeat (the control group) and the other groups; For panel c, e, k, n, o and p, Kruskal-Wallis test followed by Dunn’s multiple comparisons test was performed for statistical analysis of difference between DIO_CKrepeat (the control group) and the other groups. Data were shown as the mean ± SEM unless otherwise indicated.
