Extended Data Fig. 6: The microbiota and metabolome of caecal contents of experimental mice.
From: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids
a and b, the box-and-whiskers plots displaying alpha diversity with Shannon index (a) and Chao1 index (b); statistical method: two-sided T test/ANOVA; c and d, the pCoA plots displaying the beta diversity; distance method: Bray-Curtis Index, statistical method: ANOSIM; e and f, The histograms displaying the LDA Effect Size (LefSe) analysis results of significantly differed taxonomic compositions at known taxonomic levels (e) and amplicon sequence variant (ASV) level (f); g, The volcano plot displaying the differed metabolite compositions of intestinal contents between DIO_CM and DIO_CK mice; Two-tail T test was used for statistical analysis of the significance of fold changes (FCs). The solid circles were colored by their metabolite categories as listed in the top of the panel; the 25 significantly-changed metabolites between DIO_CM and DIO_CK groups (P value < 0.05) were listed in the panel; h, The bar chart displaying the total concentrations of each chemical class that were significantly changed in panel g; i, The bar chart displaying the concentration of TUDCA, GUDCA and T-βMCA in the caecal contents; j, The correlation network displaying the spearman correlations between the changes of ASVs and gut metabolites; The square nodes represented the significantly changed metabolites in panel g and colored in terms of their chemical class, while the circle nodes represented the significantly changed ASVs listed in panel e; enriched metabolites/ASVs: pink color, reduced metabolites/ASVs: green color; The edge thickness manifested the correlation r values (the minimal cut-off value was 0.7), and the colors displayed the positive (pink) and negative (green) correlations between ASVs and metabolites. n = 5 biologically independent samples for DIO_CK, DIO_ND and C57BL/6J groups; n = 10 biologically independent samples for DIO_CM group. For panel h and i, two-tail T test was performed for statistical analysis of difference between DIO_CK and DIO_CM group; Symbols: **** indicates P value < 0.0001, *** indicates P value < 0.001, ** indicates P value < 0.01, * indicates P value < 0.05. Data were shown as the mean ± SEM unless otherwise indicated.
