Extended Data Fig. 8: The effects of 3-O-acyl-CAs on cell cultures.
From: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids
a, The Dual-luciferase assay exhibiting the activation effect of FXR by 3-O-acyl-CAs, n = 4 biologically independent samples; b, The evaluation of cytotoxin of Ac-CA to Caco-2 cells with cell counting kit-8, n = 3 biologically independent samples; c, The evaluation of cytotoxin of Buty-CA to HEK293 cells with cell counting kit-8, n = 3 biologically independent samples; d, The evaluation of cytotoxin of Ac-CA to Caco-2 cells with cell counting kit-8, n = 3 biologically independent samples; e, The evaluation of cytotoxin of Buty-CA to Caco-2 cells with cell counting kit-8, n = 3 biologically independent samples; f, the dose–response inhibition of FXR by TβMCA, n = 3 biologically independent samples; g, the half maximal inhibitory concentrations (IC50) of TβMCA to the FXR; h, The Dual-luciferase assay exhibiting the effect of TGR5, n = 4 biologically independent samples; i and j, the distribution of CAs in germfree mice gavaged with 10 mg/kg body weight buty-CAs (g, n = 3 biologically independent samples) and in conventional C57BL/6J (h, n = 5 biologically independent samples). For panel a to f and h, one-Way ANOVA test followed by Dunnett’s multiple comparisons test was performed for statistical analysis of difference between control group (or 0 time point group) and the other groups; For panel i, Kruskal-Wallis test followed by Dunn’s multiple comparisons test was performed for statistical analysis of difference between the control group or 0 h group and the other groups. Symbols: **** indicates P value < 0.0001, *** indicates P value < 0.001, ** indicates P value < 0.01, * indicates P value < 0.05. Not significant groups were not marked in the panels. Data were shown as the mean ± SEM unless otherwise indicated.
