Extended Data Fig. 4: Pollen consumption but not colonization increases bee gut weight.
From: Host-derived organic acids enable gut colonization of the honey bee symbiont Snodgrassella alvi
Increased weights with pollen in the diet of whole bees (A) (95% CI [22.1 mg, 29.7 mg]) and dissected bee Guts (B) (95% CI [19.0 mg, 24.8 mg]) are significantly higher, but no effect was measured when colonized with S. alvi and/or Gilliamella [Bee weight, n = 271, Diet: F(1,262) = 176.1, P < .0001; S. alvi: F(1,262) = 0.1, P = .769; Gilliamella: F(1,262) = 1.0, P = .311; S. alvi:Gilliamella: F(1,262) = 0.7, P = .388], [Gut weight, n = 271, Diet: F(1,262) = 220.0, P < .0001; S. alvi: F(1,262) = 0.3, P = .595; Gilliamella: F(1,262) = 0.02, P = .880; S. alvi:Gilliamella: F(1,262) = 2.3, P = .131]. (C) The body weight, or difference between bee and gut weights, was also significantly, albeit only slightly (95% CI [0.7 mg, 7.5 mg]) higher across colonization groups for bees fed a pollen diet [Body weight, n = 271, Pollen: F(1,262) = 5.2, P = .023; S. alvi: F(1,262) = 1.4, P = .240; Gilliamella: F(1,262) = 0.6, P = .442; S. alvi:Gilliamella: F(1,262) = 3.8, P = .052]. The p values (ANOVA) are shown for the fixed effect terms in the linear mixed models fitted by ML with nested, random cage effects. The median and the IQR are depicted by the box, with whiskers extending to the furthest data points up to 1.5 xIQR. ((Pollen ±): No bacteria: n = 40/40; S. alvi: n = 36/36; S. alvi + Gilliamella: n = 36/36; Gilliamella: n = 28/27).
