Extended Data Fig. 4: Effects of metabolite supplementation on growth and population percentages of three pairs of two-member co-cultures.
From: A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities
a. Diagram of two-member cross-feeding co-culture and strain combination table; b. Maximal cell growth of three pairs of co-cultures within 72 h with/without metabolite supplementation; c. Population percentages of each member of three pairs of co-cultures at 72 h with/without metabolite supplementation. Metabolite dosages mg/L were ade 2.5, 5, 10; lys 12.5, 25, 50; his 5, 10, 20; 12.5, 25, 50; trp 10, 20, 40; leu 15, 30, 60, respectively. Ade_2.5 means the final medium contains 2.5 mg/L ade supplementation. N = three biologically independent samples, and data are presented as mean values +/− SD. One-way ANOVA, followed by Bonferroni’s multiple comparisons test with 95% confidence intervals were performed using Prism 9.5.0 (GraphPad) software, and p values were noted.
