Fig. 1: High-throughput FACS-based enrichments for mutants defective in envelope assembly. | Nature Microbiology

Fig. 1: High-throughput FACS-based enrichments for mutants defective in envelope assembly.

From: FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus

Fig. 1

a,b, Schematics showing the logic of the END (a) and CSD (b) enrichments. See text for details. c, FACS profiles monitoring light scattering (SSC height) and PI fluorescence for wild type (WT) and Δatl strains. Inset micrographs show phase contrast images with an overlay of PI staining in red (scale bar = 4 µm; the scale bar applies to all images in c). d, Schematic detailing the END enrichment workflow. An analogous CSD enrichment was performed in parallel (not shown). See text for details. Enrichment for PIHI mutants was examined by FACS profiling at each stage (top). e, The final cell populations (strain aTB015 [RN4220 ∆attB(f11)::Orf5 pTM378]) from the END and CSD sorts, as well as an ungated control, were imaged on 2% agar pads (scale bar = 4 μm; the scale bar applies to all images in e). PI staining (red) was overlaid on the phase contrast image.

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