Fig. 2: Validation and initial characterization of screening hits. | Nature Microbiology

Fig. 2: Validation and initial characterization of screening hits.

From: FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus

Fig. 2

a, Tn-seq profiles from three genomic loci showing enrichment of transposon insertions at the completion of the END and CSD sorting protocols relative to the control sort. Each vertical line represents a mapped insertion site, and the height of the line is the number of reads mapping to that site, which reflects the representation of the insertion mutant in the population. Profiles for each locus are scaled separately with the maximum number of reads indicated in the top right corner of the bottom profile. b, Representative images of WT and mutant cells (∆atl [aTP103], ∆sagB [aTB287] and ∆facZ [aTB251]) pulse labelled with sBADA to visualize PG synthesis (bottom row), stained with N,N,N-trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)-benzenaminium, 4-methylbenzenesulfonate (TMA–DPH) to label the cell membrane (‘Membrane’, middle row) and treated with PI to assess envelope permeability (‘Phase + PI’, top row). Yellow arrowheads highlight membrane and PG synthesis defects. The fluorescence intensity for each channel was scaled identically for all strains to facilitate direct comparison between images (scale bar = 4 µm; the scale bar applies to all images in b).

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