Extended Data Fig. 1: Determining the relationship between P. copri colonization efficiency and pre-colonization with B. longum subsp. infantis.

(a) Phylogenetic tree of cultured P. copri isolates used in the mouse studies described and the two MAGs positively associated with WLZ in the randomized controlled human study. The phylogenetic distance between each pair of comparisons is shown in the matrix. (b) Experimental design (n = 9 offspring per group in experiment 1 and n = 10 offspring per group in experiment 2). Mice were weaned at P28 and P25 for experiments 1 and 2, respectively. (c) Total absolute abundances of P. copri strains in fecal samples collected from mice at P42. Mean values ± SD are shown. Each dot represents a separate animal. P-values were calculated using a two-sided Mann-Whitney U test and are 4x10-4 and 3x10-4 for experiments 1 and 2, respectively.