Fig. 5: Semen EVs inhibit ZIKV infection of primary cells and tissues from the anogenital tract. | Nature Microbiology

Fig. 5: Semen EVs inhibit ZIKV infection of primary cells and tissues from the anogenital tract.

From: Phosphatidylserine-exposing extracellular vesicles in body fluids are an innate defence against apoptotic mimicry viral pathogens

Fig. 5

a, Antiviral activity of seminal plasma (SP, right panel), semen EVs and control PC-liposomes (left panel) tested against ZIKV on primary human foreskin fibroblasts. Infection determined by immunofluorescent detection of ZIKV-E and MFI measurements in microwell format (Biotek Cytation). Signals normalized to PBS-treated cells, means of n = 3 biological replicates ± standard error of the mean. Representative images (of three replicates) shown in b; scale bars, 500 μm. c, Antiviral activity of PC-liposomes (1 × 1013 particles ml−1), semen (25 vol%, equivalent to ~0.9 × 1012 particles ml−1) and semen EVs (4 × 1012 particles ml−1) tested against ZIKV in ex vivo vaginal tissue of two donors (separated in panels left and right, 32 blocks per condition, line indicates means). Two-way repeated-measures ANOVA with Bonferroni’s post-test (95% confidence interval) compares viral genome levels with PBS-treated blocks for the same timepoint; without indication P > 0.05, not significant.

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