Extended Data Fig. 7: Images related to Fig. 6 histocytometry studies. | Nature Microbiology

Extended Data Fig. 7: Images related to Fig. 6 histocytometry studies.

From: TOLLIP inhibits lipid accumulation and the integrated stress response in alveolar macrophages to control Mycobacterium tuberculosis infection

Extended Data Fig. 7

a) Representative confocal microscopy image of the lungs of a B6 mouse infected with Mtb for 56 days. Yellow – SiglecF; green – CD11b; red – pEIF2; blue – LipidTox; white — PPD. b) Histocytometry positional mapping of AM and MDM within the Mtb-infected lung. c) Proportion of pEIF2+ PPD + AM (p = 0.017 for overall ANOVA effect) and MDM (p = 0.016 for overall ANOVA effect) in B6 and Tollip−/− mice at baseline and after ISRIB treatment. p = 0.02 for overall ANOVA effect. * p < 0.05, two-sided ANOVA. N = 4 B6 control mice, N = 5 B6 ISRIB mice, N = 6 Tollip−/− control, and N = 6 Tollip−/− ISRIB mice. d) Representative images staining from the lungs of Mtb-infected B6 and Tollip−/− mice with and without ISRIB treatment. Red – pEIF2; green – CD68; blue – SiglecF; white – PPD. e) Spatial correlation analysis of cell types within 20-mm-radius neighborhoods in B6 and Tollip−/− mice. Red shades indicate positive correlation, and blue shades indicate negative correlation.

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