Extended Data Fig. 1: Tollip−/− macrophages are hyperinflammatory. | Nature Microbiology

Extended Data Fig. 1: Tollip−/− macrophages are hyperinflammatory.

From: TOLLIP inhibits lipid accumulation and the integrated stress response in alveolar macrophages to control Mycobacterium tuberculosis infection

Extended Data Fig. 1

a, b) Peritoneal extract macrophages (PEM) were isolated from Tollip−/− and B6 mice, plated, stimulated with control (Uns), LPS (10 ng/ml), PAM3 (250 ng/ml), or Mtb whole cell lysate (1 µg/ml) overnight and a) TNF and b) IL-10 were measured from cellular supernatants by ELISA. c–e) PEM were infected with Mtb H37Rv strain (MOI 2.5) overnight ex vivo, then c) TNF, d) IL-1β, and e) IL-10 were measured from cellular supernatants by ELISA. f–h) Baseline immune cell number in Tollip−/− mice. Total number of f) splenocytes, g) CD3+, C4 + CD8+, F4/80+, and NK1.1+ splenocytes, and the h) proportion of alveolar macrophages (AM) in the lungs of healthy 8-week-old mice, measured by flow cytometry. N = 3 measurements per experiment; each experiment was performed twice. * p < 0.05, student’s two sided t-test.

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