Extended Data Fig. 2: Related to Fig. 1. Characteristics of Mtb-infected Tollip−/− lungs.
a–g) Gating strategy used to identify Mtb-infected lung-resident myeloid cell subsets. a) Live/Dead Fixability dye was used to exclude dead cells, b) AM were identified by coexpression of SiglecF and CD11c. c) SiglecF- cells were gated; CD11b + Ly6G+ cells were classified as neutrophils, and CD11b + Ly6G- cells were considered macrophages. d) MHC-II+CD11c+ macrophages were subclassified as monocyte-derived macrophage cells (MDM) and MHC-II+CD11c- as interstitial macrophages (IM). e) CD11b-Ly6G- cells were gated and MHC-II+CD11c+ cells were classified as conventional DC (cDC) or f) CD103+ cDC) was measured. g) Representative flow cytometry images of the proportion of Mtb-infected myeloid subsets 28 and 56 days post infection (dpi). h–i) Proportion of AM, MDM, IM, PMN, and DC in the lungs h) 28 and i) 56 dpi. j) Representative images of Mtb-mCherry+ AM and MDM in Rag1−/− and Tollip−/−Rag1−/− mice.
