Extended Data Fig. 3: Related to Fig. 2. Gating strategy used to identify lung-resident myeloid cells in mixed bone marrow chimeric mice. | Nature Microbiology

Extended Data Fig. 3: Related to Fig. 2. Gating strategy used to identify lung-resident myeloid cells in mixed bone marrow chimeric mice.

From: TOLLIP inhibits lipid accumulation and the integrated stress response in alveolar macrophages to control Mycobacterium tuberculosis infection

Extended Data Fig. 3

a) Mixed bone marrow chimeric mice were infected with Mtb H37Rv expressing mCherry reporter plasmid (Mtb-mCherry) (50-100 CFU) via aerosol. At selected time points flow analysis was performed to identify populations infected with Mtb. Myeloid populations were identified as in Extended Data Fig. 2. a) Alveolar macrophages (AM), neutrophils (PMN), monocyte-derived macrophages (MDM), andinterstitial macrophages (IM) were subclassified as B6 or Tollip−/− based on CD45.1/CD45.2 or CD45.2 expression, respectively, and the proportion of mCherry-Mtb cells measured. Representative images from 28 days post infection are shown. b) Representative image of CD45.2+ Tollip−/− AM adoptively transferred into CD45.1+ mice 56 days after AM depletion.

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