Extended Data Fig. 7: The Type I IFN and FACT-ETS-1-depedent antiviral responses are independent pathways.
From: FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins
a-d, Representative IB (a,c) and quantification (b,d) of ETS-1 expression using IB of WCE from control or IRF3 knockout A549 cells infected with the indicated strains for 4 h (a,b) or 8 h (c,d). e-h, Representative IB (e,g) and quantification (f,h) of ETS-1 expression using IB of WCE from control or IFNAR knockout A549 cells infected with the indicated strains for 4 h (e,f) or 8 h (g,h). i-l, Representative IB (i,k) and quantification (j,l) of ETS-1 expression using IB of WCE from control or STAT1 knockout A549 cells infected with the indicated strains for 4 h (i,j) or 8 h (k,l). m, VV titers 48 hpi (MOI = 0.01) in IRF3 knockout A549 cells after treatment with indicated RNAi. Scram., scrambled. n-o, Representative IB (n) and quantification (o) of ETS-1 expression using IB of WCE from A549 cells treated with indicated doses of recombinant IFN-β for 24 h. IB of two ISGs (IFIT3 and STAT1) is also shown to illustrate ISG expression increases after IFN-β treatment. p-s, Representative IB (p,r) and quantification (q,s) of IFIT3 (ISG) expression using IB of WCE from A549 cells treated with scrambled or ETS-1 RNAi and then mock- or IFN-β (500 U/mL)-treated for 8 h (p-q) or 24 h (r-s). Data in b,d,f,h,j,l,m,o,q,s are means ± SD for n = 3 biologically independent experiments. Statistical significance was determined by unpaired two-tailed Student’s t-test. For brevity, only the most relevant statistical comparisons are shown. ns, not significant.
