Extended Data Fig. 8: A51R-hSpt16SUMO interaction suppresses ETS-1 expression and promotes VV virulence in BALB/c mice.
From: FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins
a, Representative IB of A549 WCE from cells infected with indicated VV (MOI = 10) for 4 h. b, Densitometric quantification of ETS-1 protein levels from a. Data are means ± SD; n = 3 biologically independent experiments. c, VV titers 48 hpi (MOI = 0.01) in A549 cell cultures transfected with indicated RNAi treatments. d, Percent change in body weight of BALB/c male mice still alive at each time point (n = 20 animals over two independent experiments) after intranasal VV infection (102 PFU/animal). Data are means ± SE. e, Percent survival of BALB/c mice from d over time. f, Tissue viral loads in BALB/c mice (n = 5 animals) challenged as in d 8 dpi. Horizontal lines indicate means. g, qRT-PCR analysis of relative Ets1 mRNA levels in lung tissues from BALB/c animals (n = 5) infected with 102 PFU at 8 dpi. Ets1 mRNA levels are plotted as relative to levels in mock (PBS)-treated animals. Horizontal lines indicate means. ns, not significant. Image in a is representative of at three independent experiments. Statistical significance was determined by unpaired two-tailed Student’s t-test in b and c, by Log-Rank (Mantel-Cox) tests in e, and by unpaired two-tailed Mann-Whitney U tests in f and g. Only the most relevant statistical comparisons with P < 0.05 are shown for brevity.
