Extended Data Fig. 1: The ΔA51R strain exhibits normal viral gene expression and genome replication but displays defective virion assembly in the presence of hSpt16.
From: FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins
a, IB of early (F4L, I3L) and late (F9L, A27L) in A549 WCE at indicated times post-infection (MOI = 10). IB is representative of at least two independent experiments. b, VV genome quantification by qPCR in A549 cultures at indicated times post-infection (MOI = 10). Data are means ± SD for n = 3 experiments. Statistical significance was determined by unpaired two-tailed Student’s t-test comparing genome copy number between ΔA51RFA51R and ΔA51R treatments within each time point. Only statistical comparisons with P < 0.05 are shown. c,d Analysis of total mature virus (MV) and immature virus (IV) frequencies (expressed as a percentage of total particles counted) using electron microscopy in A549 cells 24 hpi (MOI = 10) under scrambled (scram.) or hSpt16 RNAi conditions. c, Representative images of MV particles and various IV forms (crescents, spheres, spheres with nucleoid DNA) typically found in ΔA51RFA51R (top image) and ΔA51R-infected (bottom image) cells, respectively under control RNAi conditions. Selected examples of different types of MV and IV particles are indicated. Scale bar, 800 nm. d, Percentage of MV particles counted among total (MV + IV particles) particles in cells counted from each infection treatment. Data are means ± SD for n = 3 biologically independent experiments.
