Extended Data Fig. 8: Impact of SARS-CoV-2 exposure on organoid electrical activity.

(a) Micrograph of an organoid on a 3D MEA electrode, representative of at least ten organoids. Scale bar = 30 µm. (b) Extracellular electrical trace of an electrode on which a SARS-CoV-2-exposed cerebral organoid was seeded. (c) Confusion matrix for time-wise testing. The model has been trained on recordings of infected and mock condition 24 hpi, and tested on 0 hpi, 0.5 hpi and 24 hpi for SARS-CoV-2 infected and mock conditions. CUP: Confidence Upon Prediction. (d) Confusion matrix for testing the impact of Spike. The rows represent the different outputs the RFC model has been trained to recognize. The columns represent the data we provided to the model for testing purposes. The model has been trained on recordings of infected and mock condition at 24 hpi, and tested on recordings of infected, mock, and Spike-treated infected condition at 24 hpi. CUP: Confidence Upon Prediction. (E) Principal component analysis fitted on mock and infected conditions at 24 hpi, then applied on the Spike-treated infected recordings 24 hpi. The ellipses represent the confidence for each condition.