Extended Data Fig. 3: SARS-CoV-2 infection of cerebral organoids containing infiltrated monocytes.

(a) Cerebral organoids differentiated for 45 days were cocultured for 7 days with human primary monocytes and stained for Iba-1 (orange) and Dapi (purple). The micrograph is representative of fields of views originating from 2 organoids. (b) Cerebral organoids differentiated for 45 days were cocultured for 7 days with human primary monocytes and Mock infected for 10 days. The organoids were stained for CD68, CD163 or CD206 (cyan) and Iba-1 (purple). Each staining panel represents one organoid. (c) Cerebral organoids differentiated for 45 days were cocultured for 7 days in the absence or presence of human primary monocytes, infected for 3 or 10 days with SARS-CoV-2 at 105 pfu and stained for Bassoon.