Extended Data Fig. 4: SARS-CoV-2 infection of human post mortem brain slices.
From: Brain exposure to SARS-CoV-2 virions perturbs synaptic homeostasis
OPAB were mock-infected or infected with SARS-CoV-2 at 105 pfu/well. (a) Three-dimensional snapshots of a slice infected with a replication-competent SARS-CoV-2 mNeonGreen reporter virus. (b) RNA extraction and RT-qPCR were performed and the data corresponds to SARS-CoV-2 sgRNA levels normalized by GAPDH housekeeping gene from n of 3 OPAB from 2 donors. Data are presented as mean values +/− SEM. Two-tailed p value from unpaired t test was 0.0483 (*). (c-d) Infection kinetics of OPAB derived from the frontal lobe (C) and parietal lobe (D) using a replication-competent SARS-CoV-2 NanoLuciferase (NLuc) reporter virus. Each dot corresponds to a single slice and the symbols discriminate between the donors. Data are presented as mean values +/− SD, with n of 2 OPAB from 3 donors. (e) Cytotoxicity is measured for each condition from the parietal lobe from one donor using an LDH release luminescence assay. Data are presented as mean values +/− SD. nd: not determined. (f) Three-dimensional imaging of neurons (MAP2) from human brain slices exposed to SARS-CoV-2. The neurons from SARS-CoV-2-exposed brain slices retain densely aligned organization.
