Extended Data Fig. 7: Site-directed mutation of 112Tyr and 104Leu on IFITM1. | Nature Microbiology

Extended Data Fig. 7: Site-directed mutation of 112Tyr and 104Leu on IFITM1.

From: Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein–Barr virus entry into epithelial cells

Extended Data Fig. 7

(a) Schematic representation of the domain structure of EBV gH/gL; CT: C-terminal cytoplasmic tail domain, SP: signal peptide. (b) Cartoon representation of the structure of the EBV gH/gL-ligand-binding domain (LBD)41. EBV gH is indicated in green, gL is indicated in yellow, and the LBD of EphA2 is indicated in orange. (c) EphA2-overexpressing cells (NP69; HEK293) were transfected with plasmids corresponding to wild-type IFITM1 (IFITM1w), 112Tyr single-site mutation (IFITM1m1), 104Leu single-site mutation (IFITM1m2), or dual mutations (IFITM1m1+2). Experiments were implemented 72 hours after transfection. The cell lysates were harvested to test the IFITM1 level. GAPDH was used as a control to indicate equivalent amounts of lysates. The results are from at least three biological replicates.

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