Extended Data Fig. 8: IFITM1 expression is negatively regulated by YTHDF3. | Nature Microbiology

Extended Data Fig. 8: IFITM1 expression is negatively regulated by YTHDF3.

From: Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein–Barr virus entry into epithelial cells

Extended Data Fig. 8

(a) RT-qPCR shows the relative IFITM1 mRNA levels in siYTHDF1, siYTHDF2, and siYTHDF3 HEK293 cells. (b) Relative YTHDF3 mRNA levels in NP69, HK1, and HEK293 cells was checked by RT-qPCR after infecting with lentiviruses encoding shYTHDF3 or shLacZ. (c) Relative YTHDF3 mRNA levels in NP69, HK1, and HEK293 cells was checked by RT-qPCR after infecting with the control (Vector) or YTHDF3-overexpressing (YTHDF3 OE) lentiviruses. Data are presented relative to B2M (2˗ΔCt). Mean ± s.e.m., n ≥ 3 independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed t-test (a-c). (d) RT-qPCR of YTHDF3 mRNA expression in nine nasopharyngeal carcinoma (NPC) and nine nasopharyngeal (NP) samples. Total RNA from each tissue was assayed by RT-qPCR and B2M was used as a reference gene. Data are presented as mean values ± s.e.m., n = 18, two-tailed t-test. (e) Correlation analysis of the relative YTHDF3 mRNA expression level and the EBV copy number (horizontal axis: YTHDF3 relative mRNA level; vertical axis: relative EBV copy number). Data are presented as mean values ± s.e.m., n = 8, r = –0.78, p = 0.021 (e).

Source data

Back to article page