Fig. 5: The expression and function of IFITM1 were negatively regulated by YTHDF3.
a,b, IFITM1 expression levels were tested by RT−qPCR in cells from Extended Data Fig. 8b (a) and Extended Data Fig. 8c (b). Data are presented as mean ± s.e.m., n = 3 independent experiments, *P < 0.05, two-tailed t-test. c, Western blot showing the relative protein levels of YTHDF3 and IFITM1 in NP69, HK1 and HEK293 cells. The Actin protein was used as a control to indicate equivalent amounts of lysates. Representative of 2 independent experiments. d, Correlation analysis of the relative YTHDF3 and IFITM1 mRNA expression levels in 9 NPC and 9 NP samples. Data are presented as mean ± s.e.m., n = 18, r = −0.79, P = 0.00011, two-tailed t-test (d). e, TaqMan−qPCR showing the effects of YTHDF3 and IFITM1 on EBV infection. Lentiviruses encoding shYTHDF3 or shIFITM1 and their corresponding negative control lentiviruses (shLacZ + shLacZ) were transfected into NP69, HK1 and HEK293 cells. Cells were exposed to EBV-GFP for 3 h, EBV copy numbers were measured by TaqMan−qPCR and results from control groups were taken as 100%. Data are presented as mean ± s.e.m., n = 4 independent experiments, *P < 0.05, two-tailed t-test.
