Extended Data Fig. 1: IFITM1 negatively correlates with EBV infection in ECs.
(a) Heatmap of the mRNA expression levels (FPKM) of IFITMs and the reported epithelial cell receptors in B cells, EBV-negative (EBV.N) cells and EBV-positive (EBV.P) cells, analyzed by RNA sequencing. Data were plotted as the log10 of transformed cells for ease of comparison and visualization. (b) Volcano plot of the differentially expressed genes in the EBV-negative (EBVN) and EBV-positive (EBVP) groups, with upregulated genes shown in red, downregulated genes in green, and no differentially expressed genes in blue. The horizontal-axis represents −log2 (fold-change), and vertical-axis represents −log10 (p-value). n = 2 independent experiments; *p < 0.05, two-tailed t-test. (c) The Western blot analysis of tissue lysates from three NPC and three nasopharynx (NP) samples for IFITM1 protein expression using actin as a loading control, n = 3 pairs. (d) The mRNA level of IFITM1 in nine NPC and nine NP samples was assayed by RT-qPCR, and B2M was used as a reference gene, n = 9 pairs. (e) The EBV copy numbers in nine NPC and nine NP samples were also assayed by TaqMan-qPCR using an EBV detection kit, n = 9 pairs. Data are presented as mean values ± s.e.m., n = 9 pairs, *p < 0.05, **p < 0.01, ***p < 0.001 (d, e), two-tailed t-test. (f) A correlation analysis was performed between the relative IFITM1 mRNA expression and EBV copy numbers in nine NPC samples (horizontal axis: relative IFITM1 mRNA levels; vertical axis: relative EBV copy numbers). The black dot denotes scattered samples. Data are presented as mean values ± s.e.m., n = 9, r = −0.87, p = 0.0021 (f), two-tailed t-test.
