Extended Data Fig. 3: Overexpression IFITM1 inhibits EBV infection in ECs.
(a) The efficiency of overexpression of IFITM1 at the mRNA level. Bars show the relative IFITM1 mRNA level detected by RT-qPCR and data are presented relative to B2M (2˗ΔCt) and are expressed as the mean ± s.e.m. (n = 3 independent experiments, ***p < 0.001), two-tailed t-test. (b) Efficiency of overexpression of IFITM1 at the protein level. GAPDH was used as a control to indicate equivalent amounts of lysates. (c) NP69 and HEK293 cell lines were incubated with cell-free EBV-GFP for 3 hours, and the remaining extracellular viruses were removed by washing with 1×PBS. EBV copy numbers were then measured by TaqMan-qPCR. (d-g) After 72 hours, flow cytometric analyses were performed to show the percentage of EBV-GFP-positive cells. Representative images of cells infected with EBV-GFP were recorded for HEK293 cells (h). The results are expressed as the mean ± s.e.m. from at least three biological replicates. *p < 0.05, **p < 0.01 (n ≥ 3, a, b, d, e), two-tailed t-test. OE, overexpression.
