Extended Data Fig. 6: Dependence of α-1,2-fucosidase activity towards H antigens on RBCs on buffer and enzyme concentration. | Nature Microbiology

Extended Data Fig. 6: Dependence of α-1,2-fucosidase activity towards H antigens on RBCs on buffer and enzyme concentration.

From: Akkermansia muciniphila exoglycosidases target extended blood group antigens to generate ABO-universal blood

Extended Data Fig. 6

a Representative histograms of native group O RBCs stained with anti-H (BRIC231) and RAMκ-Phycoerytrhin (PE). b–d are RBCs from the same donor after treatment with of RiGH95, AmGH95A and AmGH95B (each at 1.2 µM) in conversion buffer during 60 min incubations at RT, respectively. e Overview of the data in a-d showing the median fluorescence intensity (MFI). f, g and h Same reactions as b, c and d but performed in PBS. i Overview graph of the MFI in a, f-h. j Activity of AmGH95B after 60 min incubations towards H antigens at different concentration in the presence of AmGH36A.

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