Extended Data Fig. 7: Evaluation of GH35 β-galactosidases towards the extended Gal-A antigen.
a Representative histogram (blue) of the Gal-A antigen of A1 RBCs from a single donor, compared with negative control O RBCs (dotted black line histogram), stained with anti-T/Gal-A (clone 3C9) followed by RAMκ-Phycoerythrin (PE). b and c RBCs from the same donor after treatment with AmGH35A (5.8 µM) and AmGH35B (7.2 µM), respectively, during 60 min incubations in conversion buffer at RT. d Overview of the data b and c showing the median fluorescence intensity (MFI). The dashed black line is the MFI level in the negative group O control RBCs. e Activity of AmGH35A at different concentrations in the presence of AmGH36A and AmGH95B after incubation for 30 min. For comparison, RBCs only with AmGH36A and the one-pot treatment with AmGH36A and AmGH95B are included. Logarithmic scales starting at 100 are used on the Y-axis.
