Extended Data Fig. 3: Activity of enzyme candidates towards the ExtB antigen on blood group B RBCs.
a Eleven GH20 candidates were evaluated in treatments of ExtB carrying group B RBCs from two donors (n = 2) (1 µM of each enzyme, 30 min, 38% haematocrit in conversion buffer, room temperature). The conversion was monitored by changes in mean median fluorescence intensity (MFI) of enzyme-treated and anti-ExtB-stained RBCs relative to native RBCs. b Representative flow cytometry histograms of anti-ExtB-stained blood group B RBCs (blue filled) and group O RBCs (dotted black line) as negative staining controls. c-e The RBCs from the same donors in b after treatment with AmGH20A, AmGH20C and AmGH20I, respectively. f Overview data from three group B donors (n = 3), including the example shown in panels c-e. Dotted lines in panels a and f graphs are MFI levels of group O RBC, the negative staining control. The data are the mean MFI (blue bars) of three donors (n = 3) shown as open circles.
