Fig. 2: In vivo efficacy of fluorofolin against P. aeruginosa PA14.
a, Plasma concentration of fluorofolin over time after single oral administration to neutropenic CD-1 mice. Each line is representative of an individual mouse. b, Checkerboard assay of fluorofolin and sulfamethoxazole. Z-values represent FICs determined by dividing the MIC of each drug when used in combination by the MIC when used alone. A FIC ≤ 0.5 is considered a synergistic effect. c, Treatment of mice with fluorofolin (subcutaneous) with or without SMX (100 mg kg−1, intraperitoneal). Mice were treated at 1 and 12 h post infection (n = 5 for each group). ****P ≤ 0.0001 from two-sided Tukey’s multiple comparisons test. Boxplot whiskers range from minima to maxima. Boxplots extend from the 25th to the 75th percentiles, with a line drawn at the median value for each group. d, Fluorofolin and SMX treatment of mice fed a diet of thymidine-supplemented chow during PA14 infection. Mice were treated at 1 and 12 h post infection (n = 5 for each group). ****P ≤ 0.0001 from two-sided Tukey’s multiple comparisons test. Boxplot whiskers range from minima to maxima. Boxplots extend from the 25th to the 75th percentiles, with a line drawn at the median value for each group.
