Fig. 4: CCHFV infections in human BVOs and Ldlr mutant mice.
From: Crimean–Congo haemorrhagic fever virus uses LDLR to bind and enter host cells
a, Scheme representing blood vessel organoids made from LDLR+ and LDLR− iPSC cells that were dissociated and seeded as a 2D monolayer. b, Level of infection of CCHFV (IbAr10200) BVO-derived vascular cells generated from WT and LDLR KO iPSCs. Copy numbers of CCHFV RNA were determined by RT–qPCR at 1 day post infection (d.p.i.) and 3 d.p.i. (MOI 0.1). P values were calculated using two-sided unpaired Student’s t-tests. n = 3 independent experiments. c, CCHFV (IbAr10200) infections of wild-type or Ldlr KO mice. n = 12 female mice per group (400 p.f.u.s per mouse). Numbers of CCHFV RNA copies in serum, liver and spleen of wild-type and Ldlr KO mice determined on the day of euthanasia. P values were calculated using two-sided unpaired Student’s t-tests comparing two groups. Data are mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001. d, Survival of wild-type and Ldlr KO mice. Survival analysis was done using the Kaplan–Meier test. e, Histopathological analysis (H&E staining) of livers from wild-type and some Ldlr KO mice showing little to no pathology in the Ldlr KO mice infected with CCHFV and analysed on day 4 after infection. White *, midzonal necrosis; PCN, periportal coagulative necrosis; arrows, sporadic necrosis of single cells in livers of wild-type mice. Livers of most of the Ldlr KO mice euthanized at the same time as wild-type mice showed little to no pathology. Scale bars left: 100 µm; middle and right: 20 µm. Pictures are representative of 3 mice per group. Exact P values are available in.
