Extended Data Table 3 Scoring system for mice study

From: Crimean–Congo haemorrhagic fever virus uses LDLR to bind and enter host cells

Parameter

Points

Observations

General condition

0.0

Alert, Live, active, reacts to their surroundings

0.1

Slow, weak, less active

0.4

Immobile, limited or no voluntary movement, lying motionless

The eyes

0.0

Clear and clean eyes

0.1

Slight discharge around eyes and nose

0.4

Discharge on the face and/or legs and paws. Swollen eyes

Movement and posture

0.0

Normal

0.1

Cannot fully coordinate movements

0.4

One or more of the following: Marked incoordination, hunched posture or back, lying motionless, severe lameness

Piloerection

0.0

Fur smooth and well-groomed

0.1

Mild piloerection

0.4

Severe piloerection

  1. Mice infected with CCHFV reaching the human Endpoint score fixed to 0.8 points according to Karolinska Institutet were euthanized.