Extended Data Fig. 5: Workflow for cryoEM structures of CW C-rings.
From: CryoEM structures reveal how the bacterial flagellum rotates and switches direction
(a) Workflow for the unbound CW C-ring at 4.6 Å resolution. Representative 3D reconstructions used symmetry expansion followed by local refinement. (b) Workflow for the CW C-ring with a bound partner at 5.9 Å resolution. Representative 3D reconstructions used particle subtraction followed by non-uniform refinement, symmetry expansion, and local refinement. The raw micrographs for CW rings all had thinner ice than the micrographs for CCW rings. Because of this, the MS-ring structure could not be classified in any case, and the structure could be determined without the application of particle subtraction.
