Extended Data Fig. 4: Frequency plots of primary responses to vaccination prior to diet switch.

Spleens were harvested and homogenized into single cell suspensions and 1 × 10⁶ cells were stained for CD4⁺ T cells, CD8⁺ T cells, and B220⁺ B cells at 21 days post-vaccination and quantified via flow-cytometry. Frequency of (a) CD4⁺ (CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺, p = 0.0021), (b) CD4⁺ effector memory (T_EFM, CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺ CD62L⁺ CD44⁻,) and (c) CD4⁺ central memory (T_CM, CD45_i.v.⁻ CD3⁺ CD4⁺ CD62L⁺ CD44⁺, p = 0.0007) cells. Frequency of (d) CD8⁺ (CD45_i.v.⁻ CD45⁺ CD3⁺ CD8⁺), (e) CD8⁺ T_EFM (CD45_i.v.⁻ CD45⁺ CD3⁺ CD8⁺ CD62L⁺ CD44⁻, p = 0.004), and (g) CD8⁺ T_CM (CD45_i.v.⁻ CD3⁺ CD8⁺ CD62L⁺ CD44⁺) cells. Frequency of (g) B cells (CD3⁻ B220⁺). Data are represented as boxplots as frequency of live singlets and analyzed with a two-tailed t-test used to compare groups. Note that samples were collected prior to diet switch. Corresponds to Fig. 4.

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