Extended Data Fig. 5: Recall responses are altered by HFD exposure at time of vaccination.

Lungs were harvested and homogenized into single cell suspensions and 1 × 10⁶ cells were stained for tissue resident and circulating CD4⁺ and CD8⁺ T cells at 7 days post-challenge and quantified via flow-cytometry. (a) Absolute number or (b) frequency of tissue resident CD4⁺ T (CD45_i.v.⁻ CD45⁺ CD3⁺, CD4⁺), T effector memory (T_EFM, CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺ CD62L⁺ CD44⁻) and T central memory (T_CM, CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺ CD62L⁺ CD44⁺) cells or CD8⁺ T (CD45_i.v.⁻ CD45⁺ CD3⁺ CD8⁺), T_EFM (CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺ CD62L⁺ CD44⁻), and T_CM (CD45_i.v.⁻ CD45⁺ CD3⁺ CD4⁺ CD62L⁺ CD44⁺) cells. (c) Absolute number or (d) frequency of circulating CD4⁺ T (CD45_i.v.⁺ CD45⁻ CD3⁺ CD4⁺), T effector memory (T_EFM, CD45_i.v.⁺ CD45⁻ CD3⁺ CD4⁺ CD62L⁺ CD44⁻) and T central memory (T_CM, CD45_i.v.⁺ CD45⁻ CD3⁺ CD4⁺ CD62L⁺ CD44⁺) cells or CD8⁺ T (CD45_i.v.⁺ CD45⁻ CD3⁺ CD8⁺), T_EFM (CD45_i.v.⁺ CD45⁻ CD3⁺ CD4⁺ CD62L⁺ CD44⁻), and T_CM (CD45_i.v.⁺ CD45⁻ CD3⁺ CD4⁺ CD62L⁺ CD44⁺) cells. Data include mice with obesity, always lean, and mice that were formerly obese (n = 2, n = 6, n = 4 for each group, respectively) and graphed as boxplots with comparisons made between always lean and formerly obese groups via one-way ANOVA. Frequency values are calculated as compared to live singlets.

Source data